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KMID : 0854720120320020115
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Korean Journal of Asthma, Allergy and Clinical Immunology
2012 Volume.32 No. 2 p.115 ~ p.121
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Anti-inflammatory Effect of Green Tea Cell Water in Activated Raw 264.7 Cells with Lipopolysaccharide
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Kim Min-Young
Son In-Pyeong
Kim So-Young
Song Yi-Sub
Chang Hui-Kyoung
Yoo Se-Jin
Baek Seok-Yun
Kang Hak-Hee
Kim Beom-Joon
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Abstract
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Background: The biochemical properties of green tea extracts can generally be divided into 4 aspects: antioxidative, anticarcinogenic, anti-inflammatory and antiradiation activities. Green tea extracts have 20-fold more antioxidative activity than vitamin C and also have a wide range of anti-inflammatory activity. Therefore, it is presumed that it would play a role in the treatment of chronic inflammatory disorders. Pathophysiology of immunologic disorders involves overexpression of proinflammatory mediators, including nitric oxide (NO) and prostaglandin E2 (PGE2).
Methods: After the treatment of different concentrations for Green Tea Cell Water in lipopolysaccharide (LPS)-treated Raw 264.7 cells, the levels of NO and PGE2 were measured in the media. Furthermore, we analyzed the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and phosphorylation of inhibitory kappa Bα using western blot.
Results: Green Tea Cell Water suppressed the LPS-induced expression of iNOS and COX-2 in a concentration-dependent manner. Phosphorylation of the inhibitory-κB was also inhibited.
Conclusion: Our results suggest that Green Tea Cell Water may be a significant inflammatory factor and can be used a therapeutic modality in managing chronic inflammatory diseases.
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KEYWORD
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Green Tea Cell Water, Anti-inflammation, Nitric oxide, Prostaglandins, Raw 264.7
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